Sentinel® FMDV type O Structural Protein Antibody Rapid Test could be applied on detection of the antibody against the structural protein of FMDV by the way of immune-chromatography. During the process, the specimen
(serum / plasma / whole blood) should be added to the sample well (S). The antibody labeled gold conjugate (gold-Ab) would form a complex with the antibody against structural protein of FMDV in the specimen (gold-Ab-anti-SP). When the complex overflows the test line (T), where recombinant structural protein of FMDV is immobilized, would then form a visible band. The color of the aforementioned band indicates the presence of anti-structural protein antibody in the specimen.
※ The Sentinel® FMDV type O Antibody Rapid Test should be conducted only after all materials have been brought to a temperature of 15-30℃. Also, each test should be used immediately after the sealed wrap is opened.
※ Please make sure all the components have been included. A test should not be used if the foil wrap is incomplete, or the desiccant inside of the foil wrap has turn pink in color.
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Open the sealed aluminum foil wrap.
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Add 50 μl specimen to the sample application well after by using the transfer pipette.
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Use dropper bottle to add 3 drops of running buffer to the sample application well after the specimen has been added.
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The result can be interpreted at 10 minutes after the running buffer was added.
Do not attempt to interpret results at 15 or more minutes after the running buffer was added.
INTERPRETATION OF RESULTS
When the anti-FMDV type O structural protein antibody is at or above SN 64X (Virus neutralization test, VNT), the test line (T) will show a visual test band and the appearance of such band means that the anti-FMDV type O structural protein antibody is present in the specimen.
Do not attempt to interpret results at 15 or more minutes after the running buffer was added.
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Positive: Two distinct lines (both Control Line and Test Line) appear.
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Negative: Only one line (Control Line) appears at the control area.
Invalid: If there is no line appearing at the control area, it indicates that the test is invalid, expired, or malfunctioned. In that case, please open a new foil wrap to conduct another test.
PHOTO PROCEDURES
CAUTION
The Sentinel® FMDV type O Antibody Rapid Test should be conducted only after all materials have been brought to a temperature of 15-30℃.
Each transfer pipette is only applicable for 50 μl sampling.
The operating procedures of a transfer pipette are as follows:
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Press the bulb side and place the absorbent tip of transfer pipette into the sampling tube.
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Let the capillary fill until movement stops at the end of the glass portion of tube.
* If the specimen did not fill the glass portion of the tube, press the pipette bulb twice and repeat steps 1 and 2 above.
Insert the tip into the sample application well and press the bulb side to inject the sample into the well, ensuring that all of it leaves the transfer pipette.
A. Open aluminum foil wrap before use.
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B. Uptake 50 μl specimen by transfer pipette.
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C. Add specimen to the sample application well.
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D. Use dropper bottle to add 4 drops (~100 μl) of running buffer to the sample application well after specimen is added.
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E. Result can be interpreted at 10 minutes after the running buffer was added. Do not attempt to interpret result at 15 or more minutes after the running buffer was added.