Foot and mouth disease (FMD) is a highly contagious viral disease that affects both domesticated and wild cloven-hoofed animals, such as cattle, sheep, and pigs. The incubation period for FMD may range between 1 to 7 days. In extreme cases, this period may range from as little as 12 hours to a maximum of 14 days. Animals susceptible to the FMDV infection can be characterized by a vesicular condition of the buccal mucosa, feet, and mammary glands of females.
The foot-and-mouth disease virus (FMDV) is classified within the Picornaviridae family. There are seven different serotypes: A, O, C, Asia-1, SAT-1, SAT-2, SAT-3, where each serotype has several subtypes. However, infection of one serotype does not provide immunity against any other serotypes.
The rapid replication, infection, and symptom development of the highly contagious virus means that FMD can greatly affect economies on a global scale.
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The Sentinel® FMDV NSP Ab ELISA kit includes an ELISA plate coated with monoclonal antibody and FMDV non-structural 3ABC recombinant protein.
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If FMDV NSP antibody present in serum, it will bind to 3ABC recombinant protein than HRP labeled anti-3ABC antibody bind the remaining recombinant protein.
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When reaction is stopped by TMB and stop solution, the color development is measured optically at 450nm by ELISA reader than calculating the percentage inhibition (PI) to identify existence of FMDV NSP antibody in the specimen or not. |
