The enzyme-linked immunosorbent assay (ELISA) principle is derived from the radioimmunoassay (RIA) method, which utilizes the antigen-antibody immune binding reaction. In ELISA, enzymes are conjugated to antigens or antibodies to detect their corresponding targets. By adding a substrate specific to the enzyme, the resulting color intensity is measured to evaluate the concentration of the target analyte in the specimen.

Excelsior Bio-System (EBS) customizes ELISA reagents for different products and applications through various formats, such as direct, indirect, and competitive assays. During the initial stages, EBS conducts comprehensive evaluations and screens suitable antibodies and antigens to ensure the selection of the most stable and effective raw materials.

The selected antigens and antibodies undergo purification and are conjugated with enzymes to produce ELISA reagents. Each reagent is subjected to rigorous performance validation and quality control. At the research and development stage, EBS implements thorough product validation to ensure the performance and stability of the ELISA reagents, delivering reliable and effective diagnostic solutions.